このエントリーをはてなブックマークに追加
ID 19
本文ファイル
著者
Trivedi, Subrata
Yamaguchi, Nobuo
Michibata, Hitoshi
キーワード
Ascidian
Vanadium
Metal accumulation
NDC
化学
抄録(英)
Ascidians, especially those belonging to the suborder Phlebobranchia, can accumulate high levels of vanadium. Vanadium-binding proteins (vanabins) were first isolated from a vanadium-accumulating ascidian, Ascidia sydneiensis samea, and then the vanabins were cloned, their expression was studied, and metal-binding assays were conducted. In order to unravel the mechanism of vanadium accumulation, we searched for vanabin-like genes in other animals, including other ascidians. A database search revealed five groups of cDNAs that encoded vanabin-like proteins in another ascidian, Ciona intestinalis. The genes encoding C. intestinalis vanabins, CiVanabin1 to CiVanabin5, were clustered in an 8.4-kb genomic region. The direction of transcription of each gene was identical and each gene had a single intron. All the C. intestinalis vanabins were cysteine rich, and the repetitive pattern of cysteines closely resembled that of A. sydneiensis samea vanabins. Using immobilized metal ion affinity chromatography, we found that a recombinant protein of at least one of the C. intestinalis vanabins (CiVanabin5) bound to vanadium(IV) ions.
掲載誌名
Biochimica et biophysica acta
1630巻
開始ページ
64
終了ページ
70
出版年月日
2003
出版者
Elsevier B.V.
ISSN
0167-4781
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2003 Elsevier B.V.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.bbaexp.2003.09.007
部局名
理学研究科