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ID 18
本文ファイル
著者
Yoshihara, Masao
Watanabe, Takahiro
Yamaguchi, Nobuo
Kamino, Kei
Michibata, Hitoshi
キーワード
Ascidian
Vanadium
Metal accumulation
Blood plasma
NDC
生物科学・一般生物学
抄録(英)
Some ascidians accumulate high levels of the transition metal vanadium in their blood cells. The process of vanadium accumulation has not yet been elucidated. In this report, we describe the isolation and cDNA cloning of a novel vanadium-binding protein, designated as VanabinP, from the blood plasma of the vanadium-rich ascidian, Ascidia sydneiensis samea. The predicted amino acid sequence of VanabinP was highly conserved and similar to those of other Vanabins. The N-terminus of the mature form of VanabinP was rich in basic amino acid residues. VanabinP cDNA was originally isolated from blood cells, as were the other four Vanabins. However, Western blot analysis revealed that the VanabinP protein was localized to the blood plasma and was not detectable in blood cells. RT-PCR analysis and in situ hybridization indicated that the VanabinP gene was transcribed in some cell types localized to peripheral connective tissues of the alimentary canal, muscle, blood cells, and a portion of the branchial sac. Recombinant VanabinP bound a maximum of 13 vanadium(IV) ions per molecule with a Kd of 2.8 × 10-5 M. These results suggest that VanabinP is produced in several types of cell, including blood cells, and is immediately secreted into the blood plasma where it functions as a vanadium(IV) carrier.
掲載誌名
Biochimica et Biophysica Acta
1730巻
開始ページ
206
終了ページ
217
出版年月日
2005
出版者
Elsevier B.V.
ISSN
0167-4781
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2005 Elsevier B.V.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.bbaexp.2005.07.002
部局名
理学研究科