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ID 46884
本文ファイル
著者
Mizuta, Mami
Satoh, Emi
Katoh, Chika
Tanaka, Katsuyuki
Moriguchi, Kazuki 大学院理学研究科 広大研究者総覧
Suzuki, Katsunori 大学院理学研究科 広大研究者総覧
キーワード
Horizontal gene transfer
Conjugation
Transformation
Saccharomyces cerevisiae
Interaction between bacteria and eukarya
Gene transfer from bacteria to eukarya
Transkingdom sex
Broad host range plasmid
NDC
生物科学・一般生物学
抄録(英)
Proteobacterium Escherichia coli strains harboring wide-transfer-range conjugative plasmids are able to transfer these plasmids to several yeast species. Whole plasmid DNA is mobilizable in the transkingdom conjugation phenomenon. Owing to the availability of various conjugative plasmids in bacteria, the horizontal DNA transfer has potential to occur between various bacteria and eukaryotes. In order to know host factor genes involved in such conjugation, we systematically tested the conjugability of strains among a yeast library comprising single-gene-knockout mutants in this study. This genome-wide screen identified 26 host chromosomal genes whose absence reduced the efficiency of the transkingdom conjugation. Among the 26 genes, 20 are responsible for vacuolar ATPase activity, while 5 genes (SHP1, CSG2, CCR4, NOT5, and HOF1) seem to play a role in maintaining the cell surface. Lack of either ZUO1 gene or SSZ1 gene, each of which encodes a component of the ribosome-associated cytoplasmic molecular chaperone, also strongly affected transkingdom conjugation.
内容記述
This work was supported in part by Japanese Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research (#20570221).
掲載誌名
Microbiological Research
167巻
5号
開始ページ
311
終了ページ
316
出版年月日
2012-05-20
出版者
Elsevier GmbH
ISSN
0944-5013
1618-0623
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
関連情報URL
部局名
理学研究科