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ID 28856
本文ファイル
著者
Satoh, Tetsuya
Tsuruta, Kosuke
Shinoda, Yasuharu
Hirota, Ryuichi
キーワード
ATP amplification
Reciprocating-flow system
Bioluminescence assay
NDC
生物科学・一般生物学
抄録(英)
We constructed a novel ATP amplification reactor using a reciprocating-flow system to increase the number of ATP amplification cycles without an increase in backpressure. We previously reported a continuous-flow ATIP amplification system that effectively and quantitatively amplified ATP and increased the sensitivity of a quantitative bioluminescence assay. However, it was difficult to increase the number of amplification cycles due to backpressure in the system. Because addition of immobilized adenylate kinase (ADK) and pyruvate kinase (PK) columns increased backpressure, the maximum number of ATP amplification cycles within Column durability was only 4. In this Study, ATP amplification was performed using a reciprocating-flow system, and 10 cycles of ATP amplification could be achieved without an increase in backpressure. As a result, ATP was amplified more than 100-fold after 10 cycles of reciprocating flow. The gradient of ATP amplification was approximately 1.76(N). The backpressure oil the columns was 0.03 MPa in 1-10 ATIP amplification cycles, and no increases in backpressure were observed.
掲載誌名
Analytical Biochemistry
395巻
2号
開始ページ
161
終了ページ
165
出版年月日
2009-12-15
出版者
Academic Press INC
Elsevier Science
ISSN
0003-2697
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2009 Elsevier Inc.
関連情報URL
部局名
ナノデバイス・システム研究センター