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ID 38273
本文ファイル
別タイトル
ヒト乳癌細胞株(YMB-1)の樹立とその特性
著者
Yamane, Motoi
Nishiki, Masayuki
Kishi, Naohiko
Amano, Kuniki
Nakagawa, Kenichi
Okumichi, Tsuneo
Naito, Masashi
Ito, Akihiko
Ezaki, Haruo
キーワード
Established cell line
Human breast cancer
NDC
医学
抄録(英)
A continuous cell line (YMB-1), derived from a primary breast cancer lesion has been established and maintained in culture for the past 9 months. Cell colonies consist of polygonal epithelial cells containing junctional complexes and tubular formation. The cell population doubling time is 44 hr and the saturation density at confluency is 2.5×10^5 cells/cm2. The chromosome modal number is 73 and 3 marker chromosomes are visible. Both cytosol estrogen and androgen receptors are present. However, the cytosol progesterone receptor was not detected by the dextran coated chacoal method. The s. c. injection of 5×10^6 cells from cell culuture into nude mice induced a tumor.

Both the spillage method and cell separation by specific gravity were employed to remove fibroblasts.
掲載誌名
Hiroshima Journal of Medical Sciences
33巻
4号
開始ページ
715
終了ページ
720
出版年月日
1984-12
出版者
Hiroshima University School of Medicine
ISSN
0018-2052
NCID
PubMedID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
部局名
医歯薬学総合研究科
原爆放射線医科学研究所
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