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ID 47055
本文ファイル
著者
Tamura, Takayoshi
Noda, Masafumi 大学院医歯薬保健学研究科(薬) 広大研究者総覧
Ozaki, Moeko
Maruyama, Masafumi
Matoba, Yasuyuki 大学院医歯薬保健学研究科(薬)
Kumagai, Takanori 大学院医歯薬保健学研究科(薬) 広大研究者総覧
Sugiyama, Masanori 大学院医歯薬保健学研究科(薬) 広大研究者総覧
キーワード
plant-derived lactic acid bacteria
Enterococcus avium
gamma-aminobutyric acid
NDC
医学
抄録(英)
In the present study, we successfully isolated a carrot leaf-derived lactic acid bacterium that produces gamma-aminobutyric acid (GABA) from monosodium L-glutamate (L-MSG) at a hyper conversion rate. The GABA-producing bacterium, identified as Enterococcus (E.) avium G-15, produced 115.7±6.4 g/l GABA at a conversion rate of 86.0±5.0% from the added L-MSG under the optimum culture condition by a continuous L-MSG feeding method using a jar-fermentor, suggesting that the bacterium displays a great potential ability for the commercial-level fermentation production of GABA. Using the reverse transcription polymerase chain reaction (RT-PCR) method, we analyzed the expression of genes for the GABA transporter and glutamate decarboxylase, designated gadT and gadG, respectively, which were cloned from the E. avium G-15 chromosome. Both genes were expressed even without the added L-MSG, but their expression was enhanced by the addition of L-MSG.
内容記述
This work was supported by Hiroshima Biocluster (a Cooperative Link of Unique Science and Technology for Economy Revitalization), Japan (M.S.).
掲載誌名
Biological and Pharmaceutical Bulletin
33巻
10号
開始ページ
1673
終了ページ
1679
出版年月日
2010-10-01
出版者
The Pharmaceutical Society of Japan
ISSN
0918-6158
1347-5215
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
© 2010 Pharmaceutical Society of Japan
関連情報URL
部局名
医歯薬保健学研究科