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ID 17245
本文ファイル
著者
Fujiwara, Yukichi
Kozono, Keina
Iijima, Noriaki
Nagamatsu, Yasunori
Uematasu, Kazumasa
キーワード
c-fos
immedicate early gene
goldfish
kainic acid
NDC
水産業
抄録(英)
c-fos is an immediate early gene, and is rapidly and transiently induced in neurons of the central nervous system according to their activities. To investigate neuronal activities in the brain of the goldfish (Carassius auratus), we considered that expression of c-fos mRNA would be an available marker for the neuronal activities. Therefore, we firstly isolated a cDNA clone encoding c-Fos from the goldfish brain by RT-PCR and RACE methods. A full length cDNA of the goldfish c-fos was composed of 1,044 bp open reading frame. The amino acid sequence of the goldfish c-Fos was approximately 56-90 0dentical to those of teleost fish c-Fos. Northern blot analysis showed that the expression of c-fos mRNA was rapidly and transiently induced in the brain of the goldfish by the intraperitoneal administration of kainic acid. We also showed that the identification of the c-fos mRNA expression site by in situ hybridization will be able to use as an anatomical marker for identification of the activated neuronal region in the goldfish brain.
掲載誌名
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
1巻
2号
開始ページ
253
終了ページ
259
出版年月日
2006-06
出版者
Elsevier Inc.
ISSN
1744-117X
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.cbd.2005.12.005
部局名
生物圏科学研究科