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ID 17108
本文ファイル
著者
Okuda, Jun
Arikawa, Yoko
Takeuchi, Yusuke
Mostafa, Mahmoud
Suzaki, Etsuko
Kataoka, Katsuko
Suzuki, Toshiharu
キーワード
Edwardsiella tarda
the type III secretion system
NF-кB
anti-apoptosis
intracellular replication
macrophage
NDC
生物科学・一般生物学
抄録(英)
Edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. Resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. Here we show that intracellular replication of E. tarda in murine macrophages is dependent on the type III secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic NF-κB target genes. The wild-type strain replicates within the phagosomal membrane of macrophages; whereas the type III mutant does not. Microarray analysis shows the mRNA expression level of NF-κB target genes (e.g. pro-inflammatory cytokines and anti-apoptotic genes) in macrophages infected with the wild-type strain were up-regulated compared to macrophages infected with the type III mutant. Up-regulation of Bcl2a1a, Bcl2a1b, cIAP-2, and TRAF1 genes induced expression of anti-apoptotic proteins to protect macrophages from apoptosis induced by staurosporine. Further, this protection was inhibited by adding kamebakaurin, an inhibitor of NF-κB activation and was confirmed using an NF-κB reporter gene assay. Up-regulation of anti-apoptotic NF-κB target genes is responsible for the anti-apoptotic activity of E. tarda and is required for intracellular replication in murine macrophages.
掲載誌名
Microbial Pathogenesis
41巻
6号
開始ページ
226
終了ページ
240
出版年月日
2006-12
出版者
Elsevier
ISSN
0882-4010
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier Ltd.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.micpath.2006.08.002
部局名
生物圏科学研究科