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ID 17057
本文ファイル
著者
Oku, Hiromi
Koizumi, Naoto
Okumura, Takuji
Kobayashi, Takanori
キーワード
teleost
red sea bream
Pagrus major
LPL
HL
PL
nutritional regulation
molecular cloning
NDC
水産業
抄録(英)
To investigate the nutritional regulation of lipid metabolism in fish, molecular characterization of lipases was conducted in red sea bream Pagrus major, and the effects of fasting and refeeding on their gene expression was examined. Taken together with our previous study (LPL2: Oku et al. 2002, Comp. Biochem. Physiol. 131B, 775-783), four lipase genes were identified and characterized as lipoprotein lipase (LPL), hepatic lipase (HL) and pancreatic lipase (PL). These four lipase genes, termed LPL1, LPL2, HL and PL, share a high degree of similarity with each other. Both LPL1 and LPL2 genes were expressed in various tissues including adipose tissue, gill, heart and hepatopancreas. HL gene was exclusively expressed in hepatopancreas. PL gene expression was detected in hepatopancreas and adipose tissue. Red sea bream LPL1 and LPL2 gene expression levels in hepatopancreas were increased during 48 hr fasting and decreased after refeeding, whereas no significant change in the expression levels of LPL1 and LPL2 was observed in adipose tissue, indicating that LPL1 and LPL2 gene expression is regulated in a tissue-specific manner in response to the nutritional state of fish. HL and PL gene expression was not affected by fasting and refeeding. The results of this study suggested that LPL, HL and PL gene expression is under different regulatory mechanisms in red sea bream with respect to the tissue-specificities and their nutritional regulation.
掲載誌名
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
145巻
2号
開始ページ
168
終了ページ
178
出版年月日
2006-10
出版者
Elsevier
ISSN
1096-4959
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier Ltd.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.cbpb.2006.06.008
部局名
生物圏科学研究科