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ID 20784
本文ファイル
著者
Kawanishi, Michiko
Yoshida, Teruyoyo
Kijima, Mayumi
Yagyu, Kazuhiro
Okada, Sanae
Endo, Akihito
Murakami, Masaru
Morita, Hidetoshi
Suzuki, Shoko
キーワード
Broccoli sprout
Japan
Lactococcus garvieae
Phage and pulsed-field gel electrophoresis
Phathogenicity
Radish sprouts
Seriola
NDC
産業
抄録(英)
Aims: To identify Lactococcus garvieae isolates from radish and broccoli sprouts and compare them with virulent and less virulent mutant strains obtained from yellowtails with regard to KG phenotype, presence of a capsule and virulence towards yellowtails and mice. Methods and Results: Comparative 16S rRNA gene sequence analysis of six isolates obtained from radish and broccoli sprouts indicated that they were L. garvieae (similarity >99%). They were compared with KG9502, Lg2 and ATCC49156 strains obtained from yellowtails. A less virulent mutant strain Lg2-S was obtained by Lg2 subculture. Biochemical characterization of the six strains resembled that of KG9502, Lg2, ATCC49156 and Lg2-S, except for saccharose and tagatose acidification and the presence of hippuricase. These six strains were nonpathogenic towards yellowtails and mice, nonsusceptible to bacteriophages and demonstrated heterogeneity on pulsed-field gel electrophoresis analysis. Using transmission electron microscopy, a capsule was observed in KG9502 and Lg2 but not in ATCC49156 and Lg2-S. Conclusions: We isolated L. garvieae strains that lacked pathogenicity towards yellowtails and mice from radish and broccoli sprouts; these were noncapsulated and exhibited KG+ phenotype. Significance and Impact of the Study: This is the first documentation of L. garvieae isolated from terrestrial plants. These isolates exhibited genetic diversity; however, they were noncapsulated and nonpathogenic towards yellowtails and mice.
掲載誌名
Letters in Applied Microbiology
44巻
5号
開始ページ
481
終了ページ
487
出版年月日
2007-05
出版者
Blackwell
ISSN
0266-8254
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Authors,
関連情報
The definitive version is available at www.blackwell-synergy.com
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1111/j.1472-765X.2007.02114.x
部局名
生物圏科学研究科