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ID 20750
本文ファイル
著者
Miyoshi, Miki
Fukushima, Yuji
Matsuda, Haruo
キーワード
chicken
interleukin-13 receptor
monoclonal antibody
NDC
生物科学・一般生物学
抄録(英)
Mammalian interleukin-13 (IL-13) is an important regulatory T2 cytokine secreted by activated T lymphocytes. The IL-13 receptor (IL-13R) has two different chains, IL-13Rα1 and IL-13Rα2. Although the chicken IL-13 gene is well characterized, little is known about IL-13Rs. We cloned a cDNA encoding the 380 amino acid pro-peptide of chicken IL-13Rα2 (chIL-13Rα2) and developed a monoclonal antibody (mAb), HU13-1, against it. The chIL-13Rα2 amino acid sequence showed 37-39% sequence identity with mammalian homologs. High levels of chIL-13Rα2 mRNA were expressed in liver, testis, ovary, brain, and lipopolysaccharide (LPS)-stimulated IN24 cells. HU13-1 specifically recognized recombinant chIL-13Rα2 in ELISAs, and western blots identified a 45-kDa glycoprotein or a 41-kDa non-glycosylated protein in LPSstimulated IN24 cell lysates. LPS induced a gradual increase in HU13-1-positive IN24 cells over 20 h. These results indicate that mAb HU13-1 recognizes native chIL-13Rα2 and will be valuable for further studies of chicken IL-13Rs.
掲載誌名
Developmental and Comparative Immunology
31巻
4号
開始ページ
394
終了ページ
406
出版年月日
2007
出版者
Pergamon-Elsevier Science Ltd.
ISSN
0145-305X
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier Ltd
関連情報URL
部局名
生物圏科学研究科