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ID 20744
本文ファイル
著者
Kawamoto, June-ko
Utani, Koh-ichi
キーワード
nucleolus
RNA FISH
nuclear structure and function
post-transcriptional gene regulation
c-myc
NDC
生物科学・一般生物学
抄録(英)
We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc RNA and the nucleolus. Nucleolar accumulation of c-myc RNA appeared to be temporally regulated by cell cycle progression. Hybridization with exon- and strand-specific RNA probes indicated that the non-protein coding exon 1 plays a novel role in determining the subnuclear localization of c-myc RNA. Antisense RNA targeting exon 2 localized only with nucleoplasmic foci, where it might interact with the sense strand. Thus, c-myc gene expression may be regulated by intranuclear localization of its RNA.
掲載誌名
Biochemical and Biophysical Research Communications
359巻
3号
開始ページ
806
終了ページ
810
出版年月日
2007-08-03
出版者
Academic Press Inc Elsevier Science
ISSN
0006-291X
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Elsevier Inc.
関連情報URL
部局名
生物圏科学研究科