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ID 45369
本文ファイル
著者
Nsrelden, Rehab Marray 大学院生物圏科学研究科
Horiuchi, Hiroyuki 大学院生物圏科学研究科 広大研究者総覧
Furusawa, Shuichi 大学院生物圏科学研究科 広大研究者総覧
キーワード
antimicrobial peptide
ayu
innate immunity
LPS
mRNA expression
NDC
畜産業
抄録(英)
Plecoglossus altivelis (ayu) is one of the most important fish species in the Japanese islands and in internal fish hatcheries. Living in open aquatic environments exposes fish to many pathogens. Therefore, they require rapid and strong immune defenses. We investigated in vivo the direct association between the ayu innate immune response, represented by the relative transcription of genes encoding the cathelicidin and hepcidin antimicrobial peptides, and lipopolysaccharide (LPS), a conventional pathogen-associated molecular patterns (PAMPs) of Gram-negative bacteria. Different concentrations of LPS (1, 10 and 100 µg/fish) were injected intraperitoneally into young (sexually immature) and adult (fully sexually mature) ayu. The relative expression of the antimicrobial peptide genes was measured 6 hr, 24 hr and 1 week after stimulation with LPS. We found a direct association between the expression of the antimicrobial peptide genes investigated and LPS stimulation. This relationship was time-, dose- and age-dependent. Further research is required to determine the cell-specific transcriptional regulation and posttranscriptional regulation of these antimicrobial peptides.
掲載誌名
Journal of Veterinary Medical Science
79巻
6号
開始ページ
1072
終了ページ
1080
出版年月日
2017-05-07
出版者
The Japanese Society of Veterinary Science
公益社団法人日本獣医学会
ISSN
1347-7439
0916-7250
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
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application/pdf
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権利情報
Copyright (c) 2017 The Japanese Society of Veterinary Science
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
関連情報URL
部局名
生物圏科学研究科