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ID 17107
本文ファイル
著者
Elsaied, Hosam Easa
Kaneko, Ryo
キーワード
Bathymodiolus
Deep sea
Methanotroph
RuBisCO
Symbiont
NDC
水産業
抄録(英)
In our previous investigation on the genes of 1,5-bisphosphate carboxylase/oxygenase (RuBisCO; EC 4.1.1.39) in deep-sea chemoautotrophic and methanotrophic endosymbioses, the gene encoding the large subunit of RuBisCO form I (cbbL) had been detected in the gill of a mussel belonging to the genus Bathymodiolus from a western Pacific back-arc hydrothermal vent. This study further revealed the symbiont source of the RuBisCO cbbL gene along with the genes of 16S ribosomal RNA (16S rDNA), particulate methane monooxygenase (EC 1.14.13.25; pmoA) and ATP sulfurylase (EC 2.7.7.4; sopT). The 16S rDNA sequence analysis indicated that the mussel harbors a monospecific methanotrophic Gammaproteobacterium. This was confirmed by amplification and sequencing of the methanotrophic pmoA, while thiotrophic sopT was not amplified from the same symbiotic genome DNA. Fluorescence in situ hybridization demonstrated simultaneous occurrence of the symbiont-specific 16S rDNA, cbbL and pmoA, but not sopT, in the mussel gill. This is the first molecular and visual evidence for a methanotrophic bacterial endosymbiont that bears the RuBisCO cbbL gene relevant to autotrophic CO2 fixation.
掲載誌名
Marine Biotechnology
8巻
5号
開始ページ
511
終了ページ
520
出版年月日
2006-10
出版者
Springer
ISSN
1436-2228
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlink.com"
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1007/s10126-005-6135-5
部局名
生物圏科学研究科