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ID 17059
本文ファイル
著者
Yamashita, Yusuke
Tategaki, Airo
Ogawa, Mari
Nishida, Kensho
Akita, Sachiko
Matsuda, Haruo
キーワード
chicken
chicken blastodermal cells
cytokine
ES cells
LIF
mAb
MEK1/2
STAT3
NDC
生物科学・一般生物学
抄録(英)
Leukemia-inhibitory factor (LIF) is indispensable for maintaining the undifferentiated state when propagating mouse embryonic stem (ES) cells. We previously cloned chicken LIF (chLIF) cDNA and demonstrated that it maintained chicken ES cell cultures in an undifferentiated state. Here, we developed two monoclonal antibodies, HUL-1 and HUL-2, against chLIF, which specifically recognized recombinant chLIF (rchLIF) produced by Escherichia coli and Chinese hamster ovary K1 cells, in enzyme-linked immunosorbent assays and Western blot analysis. In addition, HUL-2 inhibited the phosphorylation of signal transducer and activator of transcription 3 by rchLIF in chicken blastodermal cells (CBCs), but not that of mitogen-activated protein kinase kinase. Furthermore, the addition of HUL-2 to CBC cultures resulted in embryoid bodies forming earlier than in normal cultures. These results indicated that HUL-2 recognized not only rchLIF but also native chLIF, and suggested that CBCs in culture produce LIF, which functions in autocrine signaling.
掲載誌名
Developmental and Comparative Immunology
30巻
5号
開始ページ
513
終了ページ
522
出版年月日
2006
出版者
Elsevier
ISSN
0145-305X
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier Ltd.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.dci.2005.07.003
部局名
生物圏科学研究科