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ID 46766
本文ファイル
著者
Tajima, Takahisa 大学院先端物質科学研究科 広大研究者総覧
Fuki, Koji
Kataoka, Naoya
Kudou, Daizou
Nakashimada, Yutaka
Kato, Junichi
キーワード
Biocatalysis
Psychrophilic bacterium
3-hydroxypropionaldehyde
Biomass
抄録(英)
Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella livingstonensis Ac10 cells whose basic metabolism was inactivated by heat treatment. The 45°C heat-treated cells expressing lacZ showed maximum beta-galactosidase activity as well as chloroform/SDS-treated cells to increase membrane permeability. The fluorescent dye 5-cyano-2,3-ditolyl-tetrazolium chloride staining indicated that most basic metabolism of Ac10 was lost by heat treatment at 45˚C for 10 min. The simple biocatalyst was applied for 3-HPA production by using Klebsiella pneumoniae dhaB genes. 3-HPA was stoichiometrically produced with the complete consumption of glycerol at a high production rate of 8.85 mmol 3-HPA/g dry cell/h. The amount of 3-HPA production increased by increasing the concentrations of biocatalyst and glycerol. Furthermore, it could convert biodiesel-derived crude glycerol to 3-HPA.
内容記述
We thank Professor Tatsuo Kurihara in Kyoto University for providing S. livingstonenesis Ac10-Rifr.
掲載誌名
AMB Express
3巻
1号
開始ページ
69
出版年月日
2013-12-05
出版者
Springer Nature
ISSN
2191-0855
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
© Tajima et al.; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
関連情報URL
部局名
先端物質科学研究科