このエントリーをはてなブックマークに追加
ID 48191
本文ファイル
著者
Kumrungsee, Thanutchaporn 大学院生物圏科学研究科 広大研究者総覧
Kariya, Taishi 大学院生物圏科学研究科
Hashimoto, Kotaro 大学院生物圏科学研究科
Koyano, Takayuki
Yazawa, Nao 大学院生物圏科学研究科
Hashimoto, Takao 大学院生物圏科学研究科
Sanada, Yohei 大学院生物圏科学研究科
Matsuyama, Makoto
Sotomaru, Yusuke 自然科学研究支援開発センター 広大研究者総覧
Sakurai, Hiroaki
Loo, Fons A. J. van de
Yanaka, Noriyuki 大学院生物圏科学研究科 広大研究者総覧
抄録(英)
Tubulointerstitial fibrosis is a progressive process affecting the kidneys, causing renal failure that can be life-threatening. Thus, renal fibrosis has become a serious concern in the ageing population; however, fibrotic development cannot be diagnosed early and assessed noninvasively in both patients and experimental animal models. Here, we found that serum amyloid A3 (Saa3) expression is a potent indicator of early renal fibrosis; we also established in vivo Saa3/C/EBPβ-promoter bioluminescence imaging as a sensitive and specific tool for early detection and visualization of tubulointerstitial fibrosis. Saa3 promoter activity is specifically upregulated in parallel with tumor necrosis factor α (TNF-α) and fibrotic marker collagen I in injured kidneys. C/EBPβ, upregulated in injured kidneys and expressed in tubular epithelial cells, is essential for the increased Saa3 promoter activity in response to TNF-α, suggesting that C/EBPβ plays a crucial role in renal fibrosis development. Our model successfully enabled visualization of the suppressive effects of a citrus flavonoid derivative, glucosyl-hesperidin, on inflammation and fibrosis in kidney disease, indicating that this model could be widely used in exploring therapeutic agents for fibrotic diseases.
内容記述
This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to No. Y).
掲載誌名
Scientific Reports
9巻
開始ページ
14101
出版年月日
2019-10-01
出版者
Nature Research
ISSN
2045-2322
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
関連情報URL
部局名
生物圏科学研究科
自然科学研究支援開発センター