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ID 39962
本文ファイル
著者
Samino, Setijono
Michibata, Hitoshi
キーワード
vanadium
vanabin
metal accumulation
ascidian
NDC
生物科学・一般生物学
抄録(英)
Ascidians are known to accumulate extremely high levels of vanadium in their blood cells (up to 350 mM). The branchial sac and the intestine are thought to be the first tissues to contact the outer environment and absorb vanadium ions. The concentration of vanadium in the branchial sac and the intestine of the most vanadium-rich ascidian A. gemmata were determined to be 32.4 mM and 11.9 mM, respectively. Using an expressed sequence tag (EST) analysis of a cDNA library from the intestine of A. gemmata, we determined 960 ESTs and found 55 clones of metal-related gene orthologs, 6 redox-related orthologs, and 18 membrane transporter orthologs. Among them, 2 genes, exhibited significant similarity to the vanadium-binding proteins of other vanadium-rich ascidian species, were designated AgVanabin1 and AgVanabin2. Immobilized metal ion affinity chromatography revealed that recombinant AgVanabin1 bound to metal ions with an increasing affinity for Cu(II) > Zn(II) > Co(II), and AgVanabin2 bound to metal ions with an increasing affinity for Cu(II) > Fe(III) > V(IV). To examine the use of AgVanabins for a metal absorption system, we constructed Escherichia coli strains that expressed AgVanabin1 or AgVanabin2 fused to maltose binding protein and secreted into the periplasmic space. We found that the strain expressing AgVanabin2 accumulated about 13.5 times more Cu(II) ions than the control TB1 strain. Significant accumulation of vanadium was also observed in the AgVanabin2-expressing strain as seen by a 1.5-fold increase.
内容記述
Financial support was provided in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (#20570070 and #21570077).
掲載誌名
Mar Biotechnol
14巻
2号
開始ページ
143
終了ページ
154
出版年月日
2012-04
出版者
Springer-Verlag
ISSN
1436-2228
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) Springer Science+Business Media, LLC 2011
The final publication is available at Springer via http://dx.doi.org/10.1007/s10126-011-9396-1.
関連情報URL(IsVersionOf)
http://doi.org/10.1007/s10126-011-9396-1
部局名
理学研究科