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ID 32028
本文ファイル
著者
Shirai, Yasuhito
Morioka, Shoko
Sakuma, Megumi
Yoshino, Ken-ichi
Otsuji, Chihiro
Kashiwagi, Kaori
Chida, Kazuhiro
Shirakawa, Ryutaro
Horiuchi, Hisanori
Nishigori, Chikako
Ueyama, Takehiko
Saito, Naoaki
NDC
医学
抄録(英)
During differentiation, keratinocytes undergo a dramatic shape change from small and round to large and flat, in addition to production of proteins necessary for the formation of epidermis. It has been shown that protein kinase C (PKC) η is crucial for keratinocyte differentiation. However, its role in this process has yet to be fully elucidated. Here, we show that catalytic activity is not necessary for enlarged and flattened morphology of human keratinocytes induced by overexpression of PKCη, although it is important for gene expression of the marker proteins. In addition, we identify the small G protein RalA as a binding partner of PKCη, which binds to the C1 domain, an indispensable region for the morphological change. The binding led activation of RalA and actin depolymerization associated with keratinocyte differentiation. siRNA techniques proved that RalA is involved in not only the keratinocyte differentiation induced by PKCη overexpression but also normal keratinocyte differentiation induced by calcium and cholesterol sulfate. These results provide a new insight into the molecular mechanism of cytoskeletal regulation leading to drastic change of cell shape.
掲載誌名
Molecular biology of the cell
22巻
8号
開始ページ
1340
終了ページ
1352
出版年月日
2011-02-23
出版者
The American Society for Cell Biology
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) 2011 Shirai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution-Noncommercial-Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
関連情報URL
部局名
医歯薬学総合研究科