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ID 32185
本文ファイル
著者
Kishimoto, Akiyoshi
Hayashida, Kouichi
Kawaguchi, Hiroyuki
Abiko, Yoshimitsu
キーワード
Irsogladine maleate
Aggregatibacter actinomycetemcomitans
E-cadherin
Neutrophil migration
CXC-chemokine
Gingival epithelial cells
NDC
医学
抄録(英)
Irsogladine maleate (IM) counters Aggregatibacter actinomycetemcomitans-induced reduction of the gap junction intercellular communication and the expression of zonula occludens-1, which is a major tight junction structured protein, in cultured human gingival epithelial cells (HGEC). In addition, IM obviates the A. actinomycetemcomitans-induced increase in interleukin (IL)-8 levels in HGEC. Thus, by regulating the intercellular junctional complex and chemokine secretion in HGEC, IM may be useful to prevent periodontal disease. To clarify the effects and regulatory mechanism of IM in vivo and in vitro, we examined the expression of E-cadherin and neutrophil chemotaxis induced by A. actinomycetemcomitans under IM pretreatment. Immunohistochemical studies revealed that A. actinomycetemcomitans application to the gingival sulcus decreased the number of cells positive for E-cadherin and increased those positive for cytokine-induced neutrophil chemoattractant-2α (CINC-2α) in rat gingival epithelium.

However, in IM-pretreated rats, A. actinomycetemcomitans application had little effect on CINC-2α and E-cadherin in gingival epithelium. In cultured HGEC, real-time PCR and Western blotting showed that IM and the ERK inhibitor PD98059 abolished the A. actinomycetemcomitans-induced increase in CXCL-1 and IL-8 in HGEC. On the other hand, IM, PD98059, and the p38 MAP kinase inhibitor SB203580 recovered the decrease in E-cadherin expression. In addition, conditioned medium from A. actinomycetemcomitans-stimulated HGEC enhanced human neutrophil chemotaxis, compared to that from un-stimulated HGEC or that from A. actinomycetemcomitans-stimulated HGEC under IM pretreatment. Furthermore, IM down-regulated the p38 MAP kinase and ERK phosphorylations induced by A. actinomycetemcomitans. In conclusion, IM may control A. actinomycetemcomitans-induced gingival inflammation by regulating neutrophil migration and E-cadherin expression in gingival epithelium.
内容記述
第23回歯科基礎医学会賞受賞論文
掲載誌名
Biochemical pharmacology
79巻
10号
開始ページ
1496
終了ページ
1505
出版年月日
2010-05
出版者
Elsevier
ISSN
0006-2952
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2010 Elsevier Inc.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.bcp.2010.01.017
部局名
医歯薬学総合研究科