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ID 21548
本文ファイル
著者
Tsutsumi, Shinichi
Miyazaki, Kazuko
Pan, Haiou
Koike, Chika
Yoshida, Eri
Takagishi, Kenji
Kato, Yukio
キーワード
mesenchymal stem cell
FGF
multilineage differentiation potential
life span
NDC
医学
抄録(英)
Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF(+) MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC.
掲載誌名
Biochemical and Biophysical Research Communications
288巻
2号
開始ページ
413
終了ページ
419
出版年月日
2001-10-26
出版者
Elsevier
ISSN
0006-291X
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2001 Academic Press
関連情報URL
部局名
医歯薬学総合研究科