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ID 20576
本文ファイル
著者
Kubo-Murai, Miho
Sukenobu, Naoe
Yoshikawa, Kyoko
Inoue, Kazumi
Yamamoto, Toshiyoshi
Matsumoto, Misako
Seya, Tsukasa
Inoue, Norimitsu
キーワード
PKCδ
TLR
macrophage
signal transduction
NDC
医学
抄録(英)
Toll-like receptor (TLR) family members recognize specific molecular patterns within pathogens. Signaling through TLRs results in a proximal event that involves direct binding of adaptor proteins to the receptors. We observed that TIRAP/Mal, an adaptor protein for TLR2 and TLR4, binds protein kinase Cδ (PKCδ). TIRAP/Mal GST-fusion protein and a TIRAP/Mal antibody were able to precipitate PKCδ from rat peritoneal macrophage and THP1 cell lysates. Truncation mutants of TIRAP/Mal showed that the TIR domain of TIRAP/Mal is responsible for binding. TLR2- and TLR4-mediated phosphorylation of p38 MAPK, IKK, and IκB in RAW264.7 cells were abolished by depletion of PKCδ. These results suggest that PKCδ binding to TIRAP/Mal promotes TLR signaling events.
掲載誌名
Molecular Immunology
44巻
9号
開始ページ
2257
終了ページ
2264
出版年月日
2007-03
出版者
Pergamon-Elsevier Science Ltd.
ISSN
0161-5890
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Elsevier Ltd
関連情報URL
部局名
医歯薬学総合研究科