このエントリーをはてなブックマークに追加
ID 46814
本文ファイル
著者
Noda, Masafumi 大学院医歯薬保健学研究科(薬) 広大研究者総覧
Miyauchi, Rumi
Danshiitsoodol Narandalai 大学院医歯薬保健学研究科(薬) 広大研究者総覧
Higashikawa, Fumiko 大学院医歯薬保健学研究科(薬) 広大研究者総覧
Kumagai, Takanori 大学院医歯薬保健学研究科(薬) 広大研究者総覧
Matoba, Yasuyuki 大学院医歯薬保健学研究科(薬)
Sugiyama, Masanori 大学院医歯薬保健学研究科(薬) 広大研究者総覧
キーワード
bacteriocin
Lactobacillus brevis
actic acid bacteria
抄録(英)
In the present study, we isolated a lactic acid bacterium (LAB) from a citrus iyo fruit and identified it as Lactobacillus brevis. This plant-derived LAB strain, designated 174A, produces bacteriocin consisting of two polypeptides designated brevicin 174A-β and 174A-γ. Although each polypeptide itself displays antibacterial activity, the ability is enhanced 100 fold by mixing both polypeptides at a 1 : 1 ratio. Significantly, brevicin 174A inhibits even the growth of several pathogenic bacteria that are more resistant to a lantibiotic bacteriocin, nisin A, which is commonly utilized as a preservative added to foodstuffs. Structural analysis of the 174A bacteriocin using a program that predicts secondary structure suggests that both component polypeptides have a positively charged N-terminal region, as well as two cysteine residues in both the N- and C-terminals. Judging from a mutational analysis of the antibacterial polypeptides, these unique amino acid sequences of 174A-β might be important for the expression of the synergistic activity that occurs in the presence of the two polypeptides combined.
掲載誌名
Biological and Pharmaceutical Bulletin
38巻
12 号
開始ページ
1902
終了ページ
1909
出版年月日
2015-12-01
出版者
The Pharmaceutical Society of Japan
ISSN
0918-6158
1347-5215
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) 2015 The Pharmaceutical Society of Japan
関連情報URL
部局名
医歯薬保健学研究科