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ID 34921
本文ファイル
著者
Fujito, Naoya
Samura, Osamu
Miharu, Norio
Tanigawa, Miho
キーワード
Quantitative analysis of placental mRNA
Noninvasive prediction markers for preeclampsia
Placenta-specific 1 (PLAC1)
Glial cells-missing 1 (GCM1)
NDC
医学
抄録(英)
In this study we have investigated whether quantitative analysis of placental mRNAs in maternal plasma provides a way to monitor placental status. We measured plasma concentrations of human chorionic gonadotropin /)-subunit (/3hCG) and human placental lactogen (hPL) mRNAs as previously reported mRNAs and pregnancy associated plasma protein A (PAPP-A), placenta-specific 1 (PLACl) and glial cells-missing 1 (GCMl) mRNAs, which have not been measured during the course of normal pregnancy. Firstly, peripheral blood was obtained at various times from healthy pregnant women to clarify the time course of placental mRNAs. Secondly, blood was obtained from women with pre-eclampsia and gestational age-matched controls to examine whether placental mRNAs change in pre-eclampsia. Plasma was separated from these samples for extraction of RNA, followed by reverse transcription polymerse chain reaction analysis. Median concentrations of PLACl and GCMl mRNA in plasma of pre-eclamptic subjects respectively were 1625 and 2141 copies/ml, significantly higher than 195 and 881 copies/ml, the values for controls (Mann-Whitney test, p<0.001). No significant difference was seen in hPL, f3hCG, or PAPP-A mRNA concentration between pre-eclamptic and control groups. Plasma PLACl and GCMl mRNAs appear promising as noninvasively measurable molecular markers for pre-eclampsia.
掲載誌名
Hiroshima Journal of Medical Sciences
55巻
1号
開始ページ
9
終了ページ
15
出版年月日
2006-03
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
(c) Hiroshima University Medical Press.
部局名
医歯薬学総合研究科
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