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ID 14745
本文ファイル
著者
Yuge, Osafumi
Ninomiya, Yuichi
キーワード
22(R)-Hydroxycholesterol
Inflammation
Macrophage
22R-HC
NDC
医学
抄録(英)
Liver X receptors (LXRs) play an important role in lipid metabolism. Recently, a role for these proteins was identified in suppressing the inflammatory response. However, it is not known whether the natural ligands of LXRs, e.g. 22(R)-hydroxycholesterol (22R-HC), can suppress the inflammatory response after the onset of inflammation. We demonstrate here that treatment of Lipopolysaccharide (LPS)-activated RAW264.7 macrophages with 22R-HC markedly suppressed nitric oxide (NO) production and inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression. Additionally, 22R-HC did not affect the DNA binding activity of NF-κB, AP-1 and C/EBP(s), important transcriptional factors for iNOS and COX-2 genes expression. Furthermore iNOS and COX-2 mRNA suppression by 22R-HC was diminished by cellular treatment with cycloheximide. These results suggest that 22R-HC suppresses the expression of iNOS and COX-2 genes through de novo protein synthesis of an unidentified protein in LPS-activated macrophages. © 2005 Elsevier Ltd. All rights reserved.
掲載誌名
The Journal of Steroid Biochemistry and Molecular Biology
97巻
4号
開始ページ
376
終了ページ
383
出版年月日
2005-12
出版者
Elsevier Ltd
ISSN
0960-0760
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2005 Elsevier Ltd
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.jsbmb.2005.06.030
部局名
医歯薬学総合研究科