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ID 17222
本文ファイル
著者
Miyawaki, Yoshiyuki
Abe, Genbu
キーワード
FMRFamide
Amiloride
ENaC
Ion channel
Cloning
Aplysia
NDC
生物科学・一般生物学
抄録(英)
FMRFamide-gated Na+ channel (FaNaC) is the only known peptide-gated ion channel, which belongs to the epithelial Na+ channel/degenerin (ENaC/ DEG) family. We have cloned a putative FaNaC from the Aplysia kurodai CNS library using PCR, and examined its characteristics in Xenopus oocytes. A. kurodai FaNaC (AkFaNaC) comprised with 653 amino acids, and the sequence predicts two putative membrane domains and a large extracellular domain as in other members of the ENaC/DEG family. In oocytes expressing AkFaNaC, FMRFamide evoked amiloride-sensitive Na+ current. Different from the known FaNaCs (Helix and Helisoma FaNaCs), AkFaNaC was blocked by external Ca2+ but not by Mg2+. Also, desensitization of the current was enhanced by Mg2+ but not by Ca2+. The FMRFamide-gated current was depressed in both low and high pH. These results indicate that AkFaNaC is an FaNaC of Aplysia, and that the channel has Aplysia specific functional domains.
掲載誌名
Pflugers Archiv : European Journal of Physiology
451巻
5号
開始ページ
646
終了ページ
656
出版年月日
2006-02
出版者
Springer
ISSN
0031-6768
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlink.com"
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1007/s00424-005-1498-z
部局名
総合科学研究科