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ID 22759
本文ファイル
著者
Igarashi, Akira
Hirata, Reika
Mizuno, Noriyuki
Tsuji, Koichiro
Kawaguchi, Hiroyuki
Kato, Yukio
キーワード
human periodontal ligament cells
molecular gene marker
apolipoprotein D
MHC-DR-α
MHC-DR-β
NDC
医学
抄録(英)
Background and Objective: Molecular gene markers, which can distinguish human bone marrow mesenchymal stem cells from human fibroblasts, have recently been reported. Messenger RNA levels of tissue factor pathway inhibitor-2, major histocompatibility complex-DR-α, major histocompatibility complex-DR-β, and neuroserpin are higher in human bone marrow mesenchymal stem cells than in human fibroblasts. However, human bone marrow mesenchymal stem cells express less apolipoprotein D mRNA than human fibroblasts. Periodontal ligament cells are a heterogeneous cell population including fibroblasts, mesenchymal stem cells, and progenitor cells of osteoblasts or cementoblasts. The use of molecular markers that distinguish human bone marrow mesenchymal stem cells from human fibroblasts may provide insight into the characteristics of human periodontal ligament cells. In this study, we compared the molecular markers of human periodontal ligament cells with those of human bone marrow mesenchymal stem cells and human gingival fibroblasts.Material and Methods: The mRNA expression of the molecular gene markers was analyzed using real-time polymerase chain reaction. Statistical differences were determined with the two-sided Mann–Whitney U-test.Results: Messenger RNA levels of major histocompatibility complex-DR-α and major histocompatibility complex-DR-β were lower and higher, respectively, in human periodontal ligament cells than in human bone marrow mesenchymal stem cells or human gingival fibroblasts. Human periodontal ligament cells showed the lowest apolipoprotein D mRNA levels among the three types of cells.Conclusion: Human periodontal ligament cells may be distinguished from human bone marrow mesenchymal stem cells and human gingival fibroblasts by the genes for apolipoprotein D, major histocompatibility complex-DR-α, and major histocompatibility complex-DR-β.
掲載誌名
Journal of Periodontal Research
42巻
3号
開始ページ
283
終了ページ
286
出版年月日
2007-06
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2006 The Authors ; Journal compilation (c) 2006 Blackwell Munksgaard
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1111/j.1600-0765.2006.00944.x
部局名
医歯薬学総合研究科