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ID 29242
本文ファイル
著者
Yanase, Yuhki
Araki, Atsunori
Suzuki, Hidenori
Tsutsui, Tomoko
Kimura, Tatsuo
Okamoto, Keishi
Nakatani, Tatsuyuki
キーワード
Biosensor
Surface plasmon resonance
Living cell
Optical fiber
Mast cells
NDC
医学
抄録(英)
Surface plasmon resonance (SPR) sensors provide a useful means to study the interactions of biological molecules and the reaction of living cells on a sensor chip. However, conventional SPR sensors are bulky. expensive and complicated to use as common diagnostic equipment. In this study, we developed a relatively small and simple SPR system. using optical fibers of 250 pm diameter to detect the activation of living cells attached to the fiber tip. For this system, the core of 200 pm diameter with I cm length of an optical fiber was coated by gold film with 50 nm thickness to cause plasmon resonance. The light provided by a white LED and attenuated due to a SPR phenomenon in the sensor part was detected and analyzed using a spectrum detector. The difference in solvents with various refractive indexes and protein-bindings to the sensor tip was detected with sufficient sensitivity. Moreover, it detected a sustained increase of AR in a real-time manner, when RBL-2H3 mast cells were fixed onto the fiber tip and stimulated by an antigen. This small fiber SPR system might serve as a useful toot for various clinical examinations either within or outside the body.
掲載誌名
Biosensors & Bioelectronics
25巻
5号
開始ページ
1244
終了ページ
1247
出版年月日
2010-01-15
出版者
Elsevier Advanced Technology
ISSN
0956-5663
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2009 Elsevier B.V. All rights reserved.
関連情報URL
部局名
医歯薬学総合研究科