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ID 34737
本文ファイル
著者
Ajiraporn, Kongpol
Alisa, S.Vangnai
キーワード
organic-solvent tolerant bacteria
organic-solvent tolerant mechanisms
cell adaptation
Exiguobacterium
NDC
生物科学・一般生物学
抄録(英)
A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. Investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of the cell size lowers solvent partitioning into cells and the extrusion of solvents through the efflux system. A test using efflux pump inhibitors suggested that secondary transporters, i.e. resistance nodulation cell division (RND) and the multidrug and toxic compound extrusion (MATE) family, are involved in acetonitrile tolerance in this strain. In addition, its acetonitrile tolerance ability could be stably and significantly enhanced by repetitive growth in the presence of toxic acetonitrile. The marked acetonitrile tolerance of Exiguobacterium sp. SBH81 indicates its potential use as a host for biotechnological fermentation processes as well as bioremediation.
掲載誌名
Microbes and Environments
27巻
1号
開始ページ
30
終了ページ
35
出版年月日
2012
出版者
Japanese Society of Microbial Ecology, Department of Bioresource Science
ISSN
1342-6311
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
(c) 2012 the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM). All Rights are Reserved.
関連情報URL
部局名
先端物質科学研究科