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ID 41293
本文ファイル
著者
Yuri, Shota
Ozaki, Noriatsu 大学院工学研究院 広大研究者総覧
Ohashi, Akiyoshi 大学院工学研究院
キーワード
Anammox
Chloroflexi
MAR-FISH
phylogenetic analysis
substrate uptake pattern
NDC
建設工学・土木工学
抄録(英)
The coexistence of uncultured heterotrophic bacteria belonging to the phylum Chloroflexi has often been observed in anaerobic ammonium oxidation (anammox) reactors fed with synthetic nutrient medium without organic carbon compounds. To determine if coexisting Chloroflexi in anammox reactors scavenge organic matter derived from anammox bacterial cells, the present study was conducted to investigate the substrate uptake pattern of the uncultured Chloroflexi present in an anammox reactor and to clarify if they take up microbial products derived from anammox bacterial cells. To accomplish this, combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) was conducted. Phylogenetic analysis revealed that 36% of the clones analyzed in this study were affiliated with Chloroflexi. The sequence similarities to Anaerolinea thermophila and Caldilinea aerophila within the phylum Chloroflexi were only 81.0-88.7% and 80.3-83.8%, respectively. The uncultured Chloroflexi were found to incorporate sucrose, glucose, and N-acetyl-glucosamine. The 14C-tracing experiment revealed that the uncultured Chloroflexi were clearly MAR-positive, indicating the utilization of decaying anammox bacterial cell materials. Taken together, these results indicate that coexisting uncultured Chloroflexi in anammox reactors scavenge organic compounds derived from anammox bacterial cells.
内容記述
This research was partially supported by an Assistance for Research and Development of Construction Technology grant from the Ministry of Land, Infrastructure, Transport and Tourism, and by a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology.
掲載誌名
Water Science and Technology
66巻
12号
開始ページ
2556
終了ページ
2561
出版年月日
2012-10
出版者
IWA Publishing
ISSN
0273-1223
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) IWA Publishing 2012
関連情報URL(IsVersionOf)
http://doi.org/10.2166/wst.2012.479
部局名
工学研究科