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ID 34946
本文ファイル
著者
Kurokawa, Hiromi
Matsunaga, Aki
Yuge, Osafumi
キーワード
Propofol
β-adrenoreceptor
Phosphodiesterase-III inhibitor
Olprinone
NDC
医学
抄録(英)
Propofol has been shown to attenuate β-adrenoreceptor-mediated signal transduction in cardiomyocytes. Cyclic adenosine monophosphate (cAMP) is an essential second messenger of β-signal transduction, while olprinone, a phosphodiesterase-III inhibitor, improves poor cardiac performance by increasing cAMP levels. In the present study, we investigated the effects of olprinone toward the reducing effect of propofol on β-adrenoreceptor-mediated increases in cAMP production. First, suspensions of rat ventricular myocytes were incubated with isoproterenol or olprinone and the effects on cAMP concentrations were assessed. Next, propofol was added prior to the addition of isoproterenol or olprinone. Finally, following preincubation with propofol, isoproterenol with or without olprinone was added. Both isoproterenol and olprinone increased cAMP production in a dose-dependent manner. However, clinically relevant concentrations of olprinone (up to 10-7 M) did not cause a significant increase. Propofol (l0-7-10-4 M) attenuated isoproterenol-stimulated increases in cAMP production (decrease of 2 ± 4% - 43 ± 1%, as compared to the isoproterenol-stimulated state). However, the agent did not alter olprinone (10- 7 M)-stimulated cAMP production. Olprinone (10- 8 -10-6 M) reversed the attenuating effect of propofol (l0-5 M) toward isoproterenol (l0-7 M)-stimulated cAMP production dose-dependently (increase of 10 ± 5% - 79 ± 4% as compared to the propofolattenuated state). Our results suggest that an improvement in cardiac function is provided by olprinone when the β-adrenoreceptor-mediated signaling pathway is inhibited by propofol.
掲載誌名
Hiroshima Journal of Medical Sciences
57巻
1号
開始ページ
1
終了ページ
6
出版年月日
2008-03
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
(c) Hiroshima University Medical Press.
部局名
病院
医歯薬学総合研究科
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