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ID 34981
本文ファイル
著者
Tachibana, Noriko
Iwamoto, Takaaki
Kawamura, Toshiyuki
Masuda, Yuji
Mori, Toshio
キーワード
Translesion DNA synthesis
REV1
Antibody
NDC
医学
抄録(英)
Continuous exposure of cells to exogenous and endogenous agents produces many types of DNA damage during normal cell cycles. Post-replication repair, consisting of error-free and error-prone sub-pathways, is required for tolerance of such DNA damage. REV1 plays a crucial role in regulation of the error-prone pathway. To facilitate analysis of its cellular functions, we here generated a mouse REV1 monoclonal antibody, called D6, which also recognizes human REV1. The epitope for the antibody could be mapped between 860-877 amino acid residues of human REV1, which was located outside of the conserved catalytic domain. Although the antibody unfortunately could not specifically detect endogenous mouse and human REV1 by western blotting and immunohistochemistry, we established a method to identify endogenous human REV1 by immunoprecipitation-western blotting analysis combining D6 and separately generated polyclonal antibodies.
掲載誌名
Hiroshima Journal of Medical Sciences
59巻
3号
開始ページ
51
終了ページ
56
出版年月日
2010-09
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
(c) Hiroshima University Medical Press.
部局名
医歯薬学総合研究科
原爆放射線医科学研究所
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