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ID 34541
本文ファイル
著者
Awata, Takanori
Suzuki, Yuji
Tanabe, Katsuichiro
Hatamoto, Masashi
Ohashi, Akiyoshi
キーワード
anaerobic ammonium oxidation (anammox)
marine anammox bacteria
“Candidatus Scalindua" species
enrichment culture
FISH
phylogenetic analysis
NDC
公害・環境工学
抄録(英)
We established an enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria using an up-flow column reactor fed with artificial sea water supplemented with nitrogen and minerals and inoculated with coastal surface sediment collected from Hiroshima Bay. After 2 months of reactor operation, simultaneous removal of NH4+ and NO2- was observed, suggesting that an anammox reaction was proceeding. A total nitrogen removal rate of 2.17 g-N L-1 day-1 was attained on day 594 while the nitrogen loading rate was 3.33 g-N L-1 day-1. Phylogenetic analysis revealed that at least two dominant “Candidatus Scalindua" species were present in this reactor. Moreover, many uncultured bacteria and archaea, including candidate division or ammonia-oxidizing archaea, were present. Fluorescence in situ hybridization (FISH) revealed that anammox bacteria accounted for 85.5 ± 4.5% of the total bacteria at day 393. We also designed two oligonucleotide probes specific to each dominant “Candidatus Scalindua" species. A simultaneous FISH analysis using both probes showed that two different “Candidatus Scalindua" species were clearly recognizable and coexisted during reactor operation, although there was some variation in their abundance. The marine anammox bacteria enriched in this study have potential applications to the treatment of industrial wastewater containing high levels of ammonium and salt.
掲載誌名
Microbes and Environments
26巻
1号
開始ページ
67
終了ページ
73
出版年月日
2011
出版者
日本微生物生態学会
日本土壌微生物学会
ISSN
1342-6311
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM)
関連情報URL
部局名
工学研究科