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ID 25583
本文ファイル
著者
Oku, Hiromi
キーワード
adipocyte
cloning
differentiation
in vitro
fish
gene
PPAR
red sea bream
NDC
水産業
抄録(英)
To investigate the molecular mechanism of fish adipocyte differentiation, the three subtypes of PPAR genes (α, β and γ) were characterized in a marine teleost red sea bream (Pagrus major). The primary structures of red sea bream PPARs exhibited high degrees of similarities to their mammalian counterparts, and their gene expression was detected in various tissues including adipose tissue, heart and hepatopancreas. During the differentiation of primary cultured red sea bream adipocytes, three PPARs showed distinct expression patterns: The α subtype showed a transient increase and the β gene expression tended to increase during adipocyte differentiation whereas the gene expression level of PPARγ did not change. These results suggest that they play distinct roles in adipocyte differentiation in red sea bream. In the differentiating red sea bream adipocytes, mammalian PPAR agonists, 15-deoxy-∆12,14-prostaglandin J2, ciglitazone and fenofibrate did not show clear effects on the adipogenic gene expression. However, 2-bromopalmitate increased the PPARγ and related adipogenic gene expression levels, suggesting the γ subtype plays a central role in red sea bream adipocyte differentiation and in addition, fatty acid metabolites can be used as modulators of adipocyte function. Thus our study highlighted the roles of PPARs in fish adipocyte differentiation and provided information on the molecular mechanisms of fish adipocyte development.
掲載誌名
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
151巻
3号
開始ページ
268
終了ページ
277
出版年月日
2008-11
出版者
Elsevier Inc
ISSN
1096-4959
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2008 Elsevier Inc
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.cbpb.2008.07.007
部局名
生物圏科学研究科