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ID 17246
本文ファイル
著者
Shintaku, Koki
Yonekawa, Yuki
Takatsu, Nariaki
Yamada, Hiroshi
Hamada, Yoshiyuki
Hirota, Hiroshi
Michibata, Hitoshi
キーワード
vanadium
ascidian
metal-binding protein
protein-protein interaction
NDC
生物科学・一般生物学
抄録(英)
Several species of ascidians, the so-called tunicates, accumulate extremely high levels of vanadium ions in their blood cells. We previously identified a family of vanadium-binding proteins, named Vanabins, from blood cells and blood plasma of a vanadium-rich ascidian, Ascidia sydneiensis samea. The 3-dimensional structure of Vanabin2, the predominant vanadium-binding protein in blood cells, has been revealed, and the vanadium-binding properties of Vanabin2 have been studied in detail. Here, we used Far Western blotting to identify a novel protein that interacts with Vanabin2 from a blood cell cDNA library. The protein, named Vanabin-interacting protein 1 (VIP1), was localized in the cytoplasm of signet ring cells and giant cells. Using a two-hybrid method, we revealed that VIP1 interacted with Vanabins 1, 2, 3, and 4 but not with Vanabin P. The N-terminal domain of VIP1 was shown to be important for the interaction. Further, Vanabin1 was found to interact with all of the other Vanabins. These results suggest that VIP1 and Vanabin1 act as metal chaperones or target proteins in vanadocytes.
掲載誌名
Biochimica et Biophysica Acta - General Subjects
1770巻
6号
開始ページ
951
終了ページ
957
出版年月日
2007-06
出版者
Elsevier B.V.
ISSN
0304-4165
NCID
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2007 Elsevier B.V.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/j.bbagen.2007.02.003
部局名
理学研究科