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ID 33044
本文ファイル
著者
Takahashi, Katsunori
Imano, Ryota
Kibe, Tatsuya
Seimiya, Hiroyuki
Muramatsu, Yukiko
Kawabata, Naoki
Tanaka, Genki
Matsumoto, Yoshitake
Hiromoto, Taisuke
Koizumi, Yuka
Nakazawa, Norihiko
Yanagida, Mitsuhiro
キーワード
Telomere
Pot1
RecQ helicase
Anti-microtubule drug
NDC
生物科学・一般生物学
抄録(英)
Pot1 is a single-stranded telomere-binding protein that is conserved from fission yeast to mammals. Deletion of Schizosaccharomyces pombe pot1+ causes immediate telomere loss. S. pombe Rqh1 is a homolog of the human RecQ helicase WRN, which plays essential roles in the maintenance of genomic stability. Here, we demonstrate that a pot1Δ rqh1-hd (helicase-dead) double mutant maintains telomeres that are dependent on Rad51-mediated homologous recombination. Interestingly, the pot1Δ rqh1-hd double mutant displays a “cut" (cell untimely torn) phenotype and is sensitive to the antimicrotubule drug thiabendazole (TBZ). Moreover, the chromosome ends of the double mutant do not enter the pulsed-field electrophoresis gel. These results suggest that the entangled chromosome ends in the pot1Δ rqh1-hd double mutant inhibit chromosome segregation, signifying that Pot1 and Rqh1 are required for efficient chromosome segregation. We also found that POT1 knockdown, WRN-deficient human cells are sensitive to the antimicrotubule drug vinblastine, implying that some of the functions of S. pombe Pot1 and Rqh1 may be conserved in their respective human counterparts POT1 and WRN.
掲載誌名
Molecular and Cellular Biology
31巻
3号
開始ページ
495
終了ページ
506
出版年月日
2011-02
出版者
American Society for Microbiology
ISSN
0270-7306
NCID
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) American Society for Microbiology, Mol. Cell. Biol. February 2011 vol. 31 no. 3 495-506, doi: 10.1128/MCB.00613-10
関連情報URL
部局名
先端物質科学研究科