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ID 47737
本文ファイル
著者
Faisal, Hana Khairina Putri
Hirano, Shoko
Yamaoka, Emi
Fukazawa, Takahiro
Kanawa, Masami 自然科学研究支援開発センター 広大研究者総覧
Tanimoto, Keiji 原爆放射線医科学研究所 広大研究者総覧
Hattori, Noboru 大学院医歯薬保健学研究科(医) 広大研究者総覧
Kohno, Nobuoki 大学院医歯薬保健学研究院(医)
Ueda, Yuka 病院周産母子センター 広大研究者総覧
Sueda, Taijiro 大学院医歯薬保健学研究科(医)
Hiyama, Eiso 自然科学研究支援開発センター 広大研究者総覧
キーワード
Cell-free DNA
Droplet digital PCR
Lung adenocarcinoma
EGF
抄録(英)
Cell-free DNA (cfDNA) analysis may provide a non-invasive diagnostic approach for lung adenocarcinoma patients. Recently, droplet digital PCR (ddPCR) has been developed as a highly sensitive detection method for a low mutant allele percentage. The ddPCR detection limit for epithelial growth factor receptor (EGFR) mutations was evaluated using cell lines, NCI-H1975 for EGFR L858R point mutation and PC-9 for EGFR E746-A750del. Subsequently, detection of EGFR mutations by ddPCR was performed in tumor DNA (tDNA) and cfDNA samples of 19 lung adenocarcinoma patients whose tumor biopsies were already evaluated for EGFR mutations by clamp PCR (13 of L858R, 3 of E746-750del, and 3 of EGFR negative). In 12 cases, immunohistochemical analysis was performed to quantify the number of EGFR L858R-positive cells rate. EGFR point mutation or deletion were detected in 16 tumor DNA samples. In the measurable cfDNA samples, the rate of detection by ddPCR in cfDNA was 61.5% (8/13) for L858R and 100% (3/3) for E746-A750del. A relative correlation was found between the allele fraction (AF) of tDNA and the number of EGFR L858R-positive cells rate. No correlation was found between the AF of tDNA and AF of cfDNA. In our study, cfDNA mutation detection was not associated with clinicopathological features, but cases with high AF of cfDNA did have metastatic lesions. Our study shows that ddPCR enables cfDNA analysis for EGFR L858R and E746-A750del, with a high detection rate. Therefore, cfDNA analysis using ddPCR may complement to tumor biopsy and is beneficial for precision medicine in lung adenocarcinoma patients.
掲載誌名
Hiroshima Journal of Medical Sciences
68巻
1号
開始ページ
1
終了ページ
6
出版年月日
2019-03
出版者
Hiroshima University Medical Press
ISSN
0018-2052
NCID
言語
英語
NII資源タイプ
紀要論文
広大資料タイプ
学内刊行物(紀要等)
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
publisher
権利情報
Copyright (c) 2019 Hiroshima University Medical Press
部局名
医歯薬保健学研究科
病院
自然科学研究支援開発センター
原爆放射線医科学研究所
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