Fate of 14C-labeled microbial products derived from nitrifying bacteria in autotrophic nitrifying biofilms

Applied and Environmental Microbiology 71 巻 7 号 3987-3994 頁 2005-07 発行
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タイトル ( eng )
Fate of 14C-labeled microbial products derived from nitrifying bacteria in autotrophic nitrifying biofilms
作成者
Okabe Satoshi
Ito Tsukasa
収録物名
Applied and Environmental Microbiology
71
7
開始ページ 3987
終了ページ 3994
抄録
The cross-feeding of microbial products derived from 14C-labeled nitrifying bacteria to heterotrophic bacteria coexisting in an autotrophic nitrifying biofilm was quantitatively analyzed by using microautoradiography combined with fluorescence in situ hybridization (MAR-FISH). After only nitrifying bacteria were labeled with [14C] bicarbonate, biofilm samples were incubated with and without NH4+ as a sole energy source for 10 days. The transfer of 14C originally incorporated into nitrifying bacterial cells to heterotrophic bacteria was monitored with time by using MAR-FISH. The MAR-FISH analysis revealed that most phylogenetic groups of heterotrophic bacteria except the β-Proteobacteria showed significant uptake of 14C-labeled microbial products. In particular, the members of the Chloroflexi were strongly MAR positive in the culture without NH4+ addition, in which nitrifying bacteria tended to decay. This indicated that the members of the Chloroflexi preferentially utilized microbial products derived from mainly biomass decay. On the other hand, the members of the Cytophaga-Flavobacterium cluster gradually utilized 14C-labeled products in the culture with NH4+ addition in which nitrifying bacteria grew. This result suggested that these bacteria preferentially utilized substrate utilization-associated products of nitrifying bacteria and/or secondary metabolites of 14C-labeled structural cell components. Our results clearly demonstrated that the coexisting heterotrophic bacteria efficiently degraded and utilized dead biomass and metabolites of nitrifying bacteria, which consequently prevented accumulation of organic waste products in the biofilm.
NDC分類
建設工学・土木工学 [ 510 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
American Society for Microbiology
発行日 2005-07
権利情報
Copyright (c) American Society for Microbiology
出版タイプ Version of Record(出版社版。早期公開を含む)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0099-2240
[DOI] 10.1128/AEM.71.7.3987-3994.2005
[NCID] AA00543249
[DOI] http://dx.doi.org/10.1128/AEM.71.7.3987-3994.2005