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ID 48177
file
creator
Nishinaga, Masanori
Onizuka, Yuh-ichi
Hanafusa, Takahiro
Nomiyama, Kei-ichi
Watanabe, Daisuke
Kinashi, Toshifumi
Murashige, Toshiaki
Kunishi, Munehiro
Hori-e, Ryota
Hayashida, Yasufumi
subject
maternal estrogen
estrogen receptor
retinoic acid
hindbrain
anterior-posterior axis
vasculogenesis
medakafish
abstract
Fertilized eggs of lower vertebrates contain substantial amounts of steroidal hormones such as estrogen transferred from mother during oogenesis. However, molecular roles for maternal estrogen in the early embryonic development are largely unknown. Here we show that maternal estrogen and estrogen receptor-α modulate retinoic acid (RA) metabolism and RA-responsive gene expression in medaka embryos. Treatments with excess estradiol, an anti-estrogen (tamoxifen), overexpression or knockdown of estrogen receptor-α (ERα) resulted in misregulation of RA-related gene expression such as raldh2 (retinalaldehyde dehydrogenase), cyp26a1 (RA hydroxylase), fgf8 (fibroblast growth factor), rarα (RA receptor-α), and ahr1 (aryl hydrocarbon receptor). We propose that maternal estrogen/ERα plays a critical role in the feedback control of in vivo level of RA and that it also activates RA signaling for the development of hindbrain and vasculatures. This is the first report demonstrating that maternal estrogen supports successful embryonic development by controlling RA metabolism and signaling in early vertebrate embryos.
description
Supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
start page
1
end page
87
date of created
2019-09-18
language
eng
nii type
Others
HU type
Others
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2019 by Authors
department
Natural Science Center for Basic Research and Development
note
未発表原稿