ID 31923
file
title alternative
形質転換シロイヌナズナ植物における外来遺伝子近傍のゲノムDNAの解析に関する研究
creator
Sawasaki, Tatsuya
NDC
Botany
contents
CONTENTS
INTRODUCTION / p1
MATERIALS AND METHODS / p4
CHAPTER I STABLE TRANSFORMATION OF ARABIDOPSIS WITH THE BAR GENE USING PARTICLE BOMBARDMENT / p12
 Section1 Comparison of 3 selectable makers using changes in the number of drug-resistant calli on Arabidopsis root tissues / p12
 Section2 Regeneration of bialaphos-resistant Arabidopsis plants / p15
 Section3 Analyses of transgenic Arabidopsis plants / p17
 Section4 Analyses of morphology of transgenic Arabidopsis plants / p22
CHAPTER II CHARACTERIZATION OF JUNCTION REGIONS IN TRANSGENIC ARABIDOPSIS PLANTS OBTAINED BY PARTICLE BOMBARDMENT / p24
 Section1 Cloning of transgene and junction regions in transgenic Arabidopsis / p24
 Section2 Characterization of sequences of junction regions / p27
 Section3 In vitro binding assay of junction regions against tobacco matrices / p30
 Section4 Homology of junction regions / p35
 Section5 Characteristics of sequences of the CT junctions / p36
CHAPTER III INTEGRATION MODEL FOR THE TRANSGENE INTRODUCED BY PARTICLE BOMBARDMENT / p39
CONCLUSIONS / p42
ACKNOWLEDGMENTS / p44
REFERENCES / p45
SelfDOI
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
text version
ETD
rights
Copyright(c) by Author
relation references
・Sawasaki, T., Seki, M., Anzai, H., Irifune, K. and Morikawa, H. (1994) Stable transformation of Arabidopsis with the bar gene using particle bombardment. Transgenic Res. 3, 279-286.
・Sawasaki, T., Takahashi, M., Goshima, N. and Morikawa, H. Characterization of jucntion regions in transgenic Arabidopsis plants obtained by particle bombaredment. Gene (in press).
relation references URL
http://dx.doi.org/10.1007/s002990050432
http://dx.doi.org/10.1016/S0378-1119(98)00388-6
grantid
甲第1905号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(理学)
degreename En
Science
degreelevel
doctoral
date of granted
1998-09-21
department
Graduate School of Science