ID 31926
file
title alternative
ウニ形態形成に関わる母系因子の研究
creator
Ogawa, Mari
NDC
Zoology
abstract
Expression of genes underlying various developmental processes such as morphogenesis, body plan, etc. is regulated by cascades of transcription factors. Factors or cell fate determinants of maternal origin are supposed to play important roles to drive these cascades. Sea urchin is one of the most useful material to study maternal factors and transcriptional cascades, because of simplicity of its developmental pattern and body plan and of easiness to get synchronized embryos in a large amount. In addition, the simple and quick procedure to introduce exogenous genes into a large number of sea urchin eggs has been developed in our laboratory.

The study in the present thesis has been undertaken to identify some of the maternal factors of sea urchin eggs and to elucidate their role in sea urchin development. In Part 1 of this thesis, detection and identification of maternal factors, poly(A)+ mitochondrial ribosomal type RNA (mt 12S rRNA and mt 16S rRNA), asymmetrically enriched in small micromeres are described. These two mt rRNAs are localize outside of mitochondria and distinguished from intramitochondrial rRNAs. Based on this findings, the author speculates possible role of extramitochondrial mt rRNAs in sea urchin development.

In Part II, the author has detected other maternal factor, HpSox protein, in sea urchin eggs, and found that by binding to a Sox motif in the promoter of the arylsulfatase gene HpSox protein regulates expression of arylsulfatase protein that is related to build up body plan of pluteus larvae.
contents
Contents
General Abstract / p1
General Introduction / p3
 References / p6
Part I Asymmetrical distribution of mitochondrial rRNA into small micromeres of sea urchin embryos / p8
 Abstract / p9
 Introduction / p10
 Materials and Methods / p12
 Results / p17
 Figures / p20
 Discussion / p25
 References / p28
Part II Sox and other regulatory elements in the promoter region of the sea urchin arylsulfatase Gene / p34
 Abstract / p35
 Introduction / p36
 Materials and Methods / p38
 Results / p41
 Figures / p46
 Discussion / p52
 References / p55
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
rights
Copyright(c) by Author
relation references
Asymmetrical distribution of mitochondrial rRNA into small micromeres of sea urchin embryos. Mari Ogawa, Reiko Amikura, Koji Akasaka, Tsutomu Kinoshita, Satoru Kobayashi and Hiraku Shimada. accepted for Zoological Science
relation references URL
http://dx.doi.org/10.2108/zsj.16.445
grantid
甲第1981号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(理学)
degreename En
Physical Science
degreelevel
doctoral
date of granted
1999-03-25
department
Graduate School of Science