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ID 47694
file
creator
Sada, Naotaka
Hanafusa, Takahiro
Onizuka, Yuichi
Hayashida, Yasufumi
Kageyama, Shota
subject
arly hydrocarbon receptor
common cardinal vein
retinoic acid
retinoic acid receptor
medakafish
abstract
Retinoic acid (RA) has been shown to have a role in vascular formation, but how it affects is not fully understood. Previously, we reported that RA and its nuclear receptor (RAR) is required for transcription of ahr1 encoding an aryl hydrocarbon receptor (AHR), and that pharmacological modulation of RA, RAR, and AHR impairs the formation of common cardinal veins (CCVs) on the yolk of medakafish embryos. Here, to delineate a role for ahr1 in the vascular formation, we used an antisense-ahr1 mRNA to suppress ahr1. Following the development of vegfr1-expressing angioblast cells, we show that the antisense-ahr1 greatly inhibited the accumulation of angioblasts at the prospective branchial arch (PBA) where CCVs begin to develop on the yolk and the following CCV formation, demonstrating for the first time the essential role of ahr1 in the embryonic vascular formation of vertebrates. We also show that rarα and ahr1 mRNAs are co-expressed at PBA, suggesting a possible role of the specific expression.
description
This work was supported in part by a grant from the Ministry of Education, Science, Sports and Culture of Japan.
start page
1
end page
26
date of created
2019-06-07
language
eng
nii type
Others
HU type
Others
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2019 by Authors
department
Natural Science Center for Basic Research and Development
note
未発表原稿