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ID 39144
file
title alternative
Artificial Rearing System for Praesagittifera naikaiensis (Acoela, Acoelomorpha) <Article>
creator
Hikosaka-Katayama, Tomoe
subject
Acoelomorpha
Praesagittifera naikaiensis
Sex maturation
Development
Symbiosis
Evo–Devo
NDC
Zoology
abstract
Praesagittifera naikaiensis is an acoelomorph worm native to the seashore of the Seto Inland Sea. P. naikaiensis harbors a species of prasinophytes, Tetraselmis sp., as a symbiont. The symbiotic algae are acquired horizontally from the environment during the larval stage. In this study, we established a method for the rearing of adult P. naikaiensis as a first step toward using the animal in the study of development, evolution, and the symbiotic mechanism. We kept the animals in aquaria with closed seawater circulations. The bottom of the aquarium was half covered with sand collected from the seashore of the Seto Inland Sea. An LED light was suspended over the aquarium to aid photosynthesis. The system was maintained at a salinity of 32–33 ppt and a temperature of 14°C, on a 14-h light (35–80 μmol quanta m−2s−1)/10-h dark photoperiod. We succeeded in constantly producing sexually mature animals, independent of season. The system developed here will serve as a foundation for future studies on acoel evolution and development.
description
本研究は科学研究費補助金(26924012)の助成を受けたものである。
journal title
Bulletin of the Graduate School of Integrated Arts and Sciences, Hiroshima University. I, Studies in human sciences
volume
Volume 10
start page
17
end page
23
date of issued
2015-12-31
publisher
広島大学大学院総合科学研究科
issn
1881-7688
ncid
language
jpn
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2015 by Graduate School of Integrated Arts and Sciences, Hiroshima University All rights reserved
department
Natural Science Center for Basic Research and Development
Graduate School of Integrated Arts and Sciences
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