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ID 17058
file
creator
Oku, Hiromi
Tokuda, Masaharu
Okumura, Takuji
subject
adipocyte
stromal-vascular cells
differentiation
lipoprotein lipase
LPL
teleost
red sea bream
Pagrus major
NDC
Fishing industry. Fisheries
abstract
Various kinds of hormones including insulin, triiodothyronine (T3) and fat-soluble vitamins have been proposed as mediators of adipocyte differentiation in mammals. To investigate the factors which are responsible for fish adipocyte differentiation, we developed a serum-free culture system of stromal-vascular cells of red sea bream adipose tissue and examined the effects of insulin (bovine), T3, and fat-soluble vitamins (all-trans retinoic acid, retinyl acetate and 1,25-dihydroxyvitamin D3) on the differentiation-linked expression of the lipoprotein lipase (LPL) gene. As assessed by the increase in LPL gene expression after 3 day cultivation, like in mammalian adipocytes, insulin enhanced the adipocyte differentiation in a concentration-dependent manner. During 2 week cultivation, bovine insulin promoted lipid accumulation in differentiating adipocytes concentration-dependently until the terminal differentiation. These results indicate that the differentiation of fish adipocytes is inducible by insulin alone. T3 alone had no effect but enhanced the differentiation-linked LPL gene expression in the presence of insulin. Fat-soluble vitamins, unlike in mammalian adipocytes, did not show any significant effects. The method developed in this study should be of interest for the characterization of factors involved in fish adipocyte differentiation.
journal title
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
volume
Volume 144
issue
Issue 3
start page
326
end page
333
date of issued
2006-07
publisher
Elsevier
issn
1096-4959
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd.
relation is version of URL
http://dx.doi.org/10.1016/j.cbpb.2006.03.008
department
Graduate School of Biosphere Science