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ID 305
file
creator
Kashiwagi, Keiko
NDC
Zoology
abstract
Gynogenetic diploids were produced from eggs of 10 female Rana rugosa by refrigeration after insemination with UV-irradiated sperm of 10 males of the same species. They were compared with the control diploids and gynogenetic haploids in developmental capacity. When refrigerated at 0∿2℃ for two hours after insemination with UV-irradiated sperm, 73.10f the eggs cleaved normally, and 65.1 0x1.5443cp+823nd 20.916032271143f these normally cleaved eggs hatched normally and became normally metamorphosed frogs, respectively. The diploidy of the individuals in the experimental series was confirmed by counting chromosomes at the tadpole stage. Of the 457 gynogenetic diploids, 418 were females and 39 (8.5%) were males. There were 931 females and 25 (2.6%) males among the 956 offspring of six gynogenetic diploid males mated with normal females. As the great majority of gynogenetic diploids and the offspring of gynogenetic males mated with normal females were females, it is assumed that the male is heterogametic in Rana rugosa and that gynogenetic males are genetic females. In the gynogenetic diploid tadpoles, there were 26 (2.4%) black-eyed and 23 (2.1%) gray-eyed mutants.
journal title
Scientific report of the Laboratory for Amphibian Biology
volume
Volume 12
start page
1
end page
22
date of issued
1993-03
publisher
Institute for Amphibian Biology, Hiroshima University
contributor
国立情報学研究所
date of created
2006-03-21
issn
0386-3166
ncid
SelfDOI
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Science
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