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ID 19
file
creator
Trivedi, Subrata
Yamaguchi, Nobuo
Michibata, Hitoshi
subject
Ascidian
Vanadium
Metal accumulation
NDC
Chemistry
abstract
Ascidians, especially those belonging to the suborder Phlebobranchia, can accumulate high levels of vanadium. Vanadium-binding proteins (vanabins) were first isolated from a vanadium-accumulating ascidian, Ascidia sydneiensis samea, and then the vanabins were cloned, their expression was studied, and metal-binding assays were conducted. In order to unravel the mechanism of vanadium accumulation, we searched for vanabin-like genes in other animals, including other ascidians. A database search revealed five groups of cDNAs that encoded vanabin-like proteins in another ascidian, Ciona intestinalis. The genes encoding C. intestinalis vanabins, CiVanabin1 to CiVanabin5, were clustered in an 8.4-kb genomic region. The direction of transcription of each gene was identical and each gene had a single intron. All the C. intestinalis vanabins were cysteine rich, and the repetitive pattern of cysteines closely resembled that of A. sydneiensis samea vanabins. Using immobilized metal ion affinity chromatography, we found that a recombinant protein of at least one of the C. intestinalis vanabins (CiVanabin5) bound to vanadium(IV) ions.
journal title
Biochimica et biophysica acta
volume
Volume 1630
start page
64
end page
70
date of issued
2003
publisher
Elsevier B.V.
issn
0167-4781
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2003 Elsevier B.V.
relation is version of URL
http://dx.doi.org/10.1016/j.bbaexp.2003.09.007
department
Graduate School of Science