このエントリーをはてなブックマークに追加
ID 18
file
creator
Yoshihara, Masao
Watanabe, Takahiro
Yamaguchi, Nobuo
Kamino, Kei
Michibata, Hitoshi
subject
Ascidian
Vanadium
Metal accumulation
Blood plasma
NDC
Biology
abstract
Some ascidians accumulate high levels of the transition metal vanadium in their blood cells. The process of vanadium accumulation has not yet been elucidated. In this report, we describe the isolation and cDNA cloning of a novel vanadium-binding protein, designated as VanabinP, from the blood plasma of the vanadium-rich ascidian, Ascidia sydneiensis samea. The predicted amino acid sequence of VanabinP was highly conserved and similar to those of other Vanabins. The N-terminus of the mature form of VanabinP was rich in basic amino acid residues. VanabinP cDNA was originally isolated from blood cells, as were the other four Vanabins. However, Western blot analysis revealed that the VanabinP protein was localized to the blood plasma and was not detectable in blood cells. RT-PCR analysis and in situ hybridization indicated that the VanabinP gene was transcribed in some cell types localized to peripheral connective tissues of the alimentary canal, muscle, blood cells, and a portion of the branchial sac. Recombinant VanabinP bound a maximum of 13 vanadium(IV) ions per molecule with a Kd of 2.8 × 10-5 M. These results suggest that VanabinP is produced in several types of cell, including blood cells, and is immediately secreted into the blood plasma where it functions as a vanadium(IV) carrier.
journal title
Biochimica et Biophysica Acta
volume
Volume 1730
start page
206
end page
217
date of issued
2005
publisher
Elsevier B.V.
issn
0167-4781
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2005 Elsevier B.V.
relation is version of URL
http://dx.doi.org/10.1016/j.bbaexp.2005.07.002
department
Graduate School of Science