このエントリーをはてなブックマークに追加
ID 30119
title alternative
モノリス型カラムを用いた尿中メタンフェタミンの抽出
creator
Nakamoto, Akihiro
Nishida, Manami
Yashiki, Mikio
Kuramoto, Takako
Takei, Yoshiyuki
Furuno, Masahiro
Minakuchi, Hiroyoshi
Nakanishi, Kazuki
Kimura, Kojiro
subject
Methamphetamine
Monolithic silica capillary column
Solid-phase extraction
Spot test
Simon's reaction
NDC
Medical sciences
abstract
We have established a simple screening method for methamphetamine (MA) in urine using a monolithic silica capillary column for extraction and using the Simon's reagent for colorimetric determination. Urine was mixed with borate buffer solution (50 mM, pH 10.2) and the mixture was passed through the column connected with the needle of a gas-tight syringe. MA in urine was adsorbed to the column, then eluted with 10 µl of ethyl acetate, and directly spotted on a thin-layer chromatographic plate by dividing the eluate into several aliquots. To separate MA from urine impurities and detect it clearly, a 2-µl volume of a solvent mixture (chloroform : methanol = 95 : 5, v/v, saturated with 28-0x1.fa060bfff8bbp+0mmonium hydroxide) was applied to the spot for concentrically development of the analyte.

After drying the solvent, 0.5% sodium nitroprusside solution containing 10% sodium carbonate was sprayed on the plate. The plate was put in a bottle saturated with acetaldehyde, and the ring was changed purple (Simon's reaction) when MA existed in urine. The macroscopic detection limit of MA in urine by this method was 0.5 µg/ml. To confirm efficiency of this method, the eluate from the column was analyzed by GC/MS using penta-deuterated MA as internal standard. By analysis of 30 urine samples, the results obtained by the present spot test agreed well with those by GC/MS.
journal title
Japanese journal of forensic toxicology
volume
Volume 22
issue
Issue 3
start page
200
end page
204
date of issued
2004
publisher
Japanese Association of Forensic Toxicology
日本法中毒学会
issn
0915-9606
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
(c) 2004 Japanese Association of Forensic Toxicology.
relation url
department
Graduate School of Biomedical Science