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ID 33079
file
title alternative
Artifi cial rearing and oviposition of Waminoa sp. (Acoela, Acoelomorpha) : Toward the development of a model system to study animal-algal symbiosis. <Articles>
creator
Hikosaka-Katayama, Tomoe
subject
Symbiosis
Acoelomorpha
Symbiodinium
Amphidinium
oogenesis
oviposition
NDC
Biology
abstract
Waminoa sp. is an acoelomorph worm that infests corals and harbors two species of dinofl agellates, Amphidinium sp. and Symbiodinium sp.. These symbiotic algae are inherited vertically from parent to offspring during oogenesis. In this study, we established a method for artificial breeding of Waminoa sp., as the first step toward using Waminoa sp. in the study of the mechanism underlying animal–algal symbiosis and the evolution of this symbiosis. We developed a system in which an aquarium circulated approximately 80 L of artifi cial seawater between an upper aquarium and a lower sump, each of which was 45 cm × 30 cm × 35 cm in size and contained 40 L of seawater. Water flowed into the upper aquarium from the sump alternately through a pipe outlet. The upper aquarium contained the hard coral Symphyllia valenciennesii, live rocks, and a layer of fine sand. A 150-W metal halide lamp was suspended over the aquarium to aid photosynthesis. The system was maintained at a salinity of 32–33 ppt and a temperature of 23°C, on a 14-h light (17–50 μmol quanta m-2s-1) : 10-h dark photoperiod. We succeeded in producing almost every stage of the life cycle of Waminoa sp., including the zygote, embryo, larva, juvenile, and adult stages, independent of seasons. In addition, we developed an artificial oviposition procedure that involved isolation from coral in the mature stage. The system developed in this study will serve as a foundation for future studies on acoel–algal symbiosis.
journal title
Bulletin of the Graduate School of Integrated Arts and Sciences, Hiroshima University. I, Studies in human sciences
volume
Volume 5
start page
39
end page
46
date of issued
2010-12-31
publisher
広島大学大学院総合科学研究科
issn
1881-7688
ncid
SelfDOI
language
jpn
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2010 by Graduate School of Integrated Arts and Sciences, Hiroshima University All rights reserved
department
Graduate School of Integrated Arts and Sciences
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