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ID 25972
file
creator
Kondo, Komei
Yamada, Keizo
Nakagawa, Ayami
Morikawa, Hiromichi
subject
Germin-like protein
GLP
nitrogen dioxide
nitric oxide
reactive nitrogen species
superoxide dismutase
reactive oxygen species
NDC
Biology
abstract
Atmospheric nitrogen dioxide (NO2) is an environmental oxidant that is removed through direct uptake by foliage, but plant responses to this highly reactive gas are not well understood at the molecular level. From NO2-exposed leaves of a woody azalea (Rhododendron mucronatum), we cloned two cDNAs (RmGLP1 and RmGLP2) for germin-like proteins (GLPs), a group of ubiquitous plant proteins that have been implicated in various plant physiological and developmental processes. Quantitative analysis of mRNA expression, together with immunoblotting data, showed that foliar exposure to NO2 caused a robust induction of these GLP-encoding genes. When produced in tobacco cell culture, recombinant RmGLP2 was secreted into the apoplast, where it exhibited superoxide dismutase activity. RmGLP1 and RmGLP2 represent the first examples of plant genes that are responsive to airborne NO2. These enzymes might have a potential role in extracellular defense mechanisms through attenuation of interactions between reactive nitrogen and oxygen species.
journal title
Biochemical and Biophysical Research Communications
volume
Volume 377
issue
Issue 3
start page
857
end page
861
date of issued
2008-12-18
publisher
Academic Press Inc.
Elsevier Science
issn
0006-291X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2008 Elsevier Inc.
relation url
department
Graduate School of Science